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KMID : 1234420080360040307
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 4 p.307 ~ p.313
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Production of Xylooligosaccharides by Yeast Cell Surface-Displayed Endoxylanase
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Kim Hyun-Jin
Lee Jae-Hyung
Kim Yeon-Hee
Nam Soo-Wan
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Abstract
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The yeast surface expression system, pCTXYN (6.8 kb), of Bacillus endoxylanase gene (xynB, 642 bp) was constructed and introduced into Saccharomyces cerevisiae EBY100 cell. The transformed yeast cell showing the highest endoxylanase activity was selected through the active staining of colonies grown on YPDG medium containing xylan. With the yeast transformant, EBY100/pCTXYN, grown on galactose containing medium, it was found that the endoxylanase was successfully displayed on the yeast cell surface and the xylooligosaccharides were efficiently produced from xylan. The most of endoxylanase activity was detected in the cell fraction and reached about 1.9 unit/mL after 48 h cultivation. The optimized conditions for xylooligosaccharides production from xylan were determined as follows: substrate and its concentration, oat spelt xylan 6%; concentration of yeast whole-cell, 5 unit/mL; temperature, 50oC, and reaction time 2~4 h. When the oat spelts xylan and corncob xylan were hydrolyzed by treatment with cell surface-displayed endoxylanase, xylotriose was formed as a main product.
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KEYWORD
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Cell surface display, Saccharomyces cerevisiae, xylan, endoxylanase, xylooligosaccharides
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